Chapter Nineteen: Metabolic Acidosis, part 3

Edited by Nayan Arora

References

Chapter 19, Part 3 August 30, 2023

  1. Joel and Roger mentioned the most common cause seems to be Sjögren’s syndrome for an acquired distal RTA. We mentioned this in an earlier episode and referenced this example of an absence of the H+ ATPase, presumably from autoantibodies to this transporter. Here’s a case report: Absence of H(+)-ATPase in cortical collecting tubules of a patient with Sjogren's syndrome and distal renal tubular acidosis

  2.  Joel mentioned this paper in the New England Journal of Medicine in which there were patients who had hyperkalemia with a distal RTA: Hyperkalemic Distal Renal Tubular Acidosis Associated with Obstructive Uropathy | NEJM in this setting, some patients 

  3.  Anna mentioned this article on “ampho-terrible:” It’s the holes!!!    Yano T, Itoh Y, Kawamura E, Maeda A, Egashira N, Nishida M, Kurose H, Oishi R. Amphotericin B-induced renal tubular cell injury is mediated by Na+ Influx through ion-permeable pores and subsequent activation of mitogen-activated protein kinases and elevation of intracellular Ca2+ concentration. Antimicrob Agents Chemother. 2009 Apr;53(4):1420-6

  4. Josh mentioned this study on furosemide’s effect on the TAL: Furosemide-induced urinary acidification is caused by pronounced H+ secretion in the thick ascending limb 

  5. Urinary acidification assessed by simultaneous furosemide and fludrocortisone treatment: an alternative to ammonium chloride - Kidney International

  6. Melanie mentioned treatment of patients with cystinosis Expert guidance on the multidisciplinary management of cystinosis in adolescent and adult patients | Clinical Kidney Journal | Oxford Academic

  7. Amy shared her observations regarding base supplements including Prevention of recurrent calcium stone formation with potassium citrate therapy in patients with distal renal tubular acidosis - PubMed and Dosage of potassium citrate in the correction of urinary abnormalities in pediatric distal renal tubular acidosis patients - PubMed

  8. Roger mentioned that he has had good luck with Moonstone Nutrition drinks alkali citrates for kidney health

  9. We referred to David Goldfarb’s teaching on kidney stones in patients with acidification defects:  A Woman with Recurrent Calcium Phosphate Kidney Stones (we also referenced this in an earlier episode but this one is a fan favorite). 

  10. Joel mentioned the concern of bone loss in distal RTA: Incomplete renal tubular acidosis in 'primary' osteoporosis and Abnormal distal renal tubular acidification in patients with low bone mass: prevalence and impact of alkali treatment

  11. JC mentioned Ehlers-Danlos syndrome with renal tubular acidosis and medullary sponge kidneys. A report of a case and studies of renal acidification in other patients with the Ehlers-Danlos syndrome

  12. Lety mentioned concerns of encrustation of stents in stone forming individuals Potassium Citrate as a Preventive Treatment for Double-J Stent Encrustation: A Randomized Clinical Trial

  13. Joel schooled us in toluene and the presentation which appears to be an RTA- https://journals.lww.com/JASN/Abstract/1991/02000/Glue_sniffing_and_distal_renal_tubular_acidosis_.3.aspx

  14. Melanie mentioned this work by Alan Yu’s lab on a mechanism of hypercalciuria Claudin-2 deficiency associates with hypercalciuria in mice and human kidney stone disease

  15. Furosemide/Fludrocortisone Test and Clinical Parameters to Diagnose Incomplete Distal Renal Tubular Acidosis in Kidney Stone Formers and an accompanying editorial by Goldfarb Refining Diagnostic Approaches in Nephrolithiasis: Incomplete Distal Renal Tubular Acidosis

  16. Here’s a nice piece on ifosfamide and phosphate from Josh New clues for nephrotoxicity induced by ifosfamide: preferential renal uptake via the human organic cation transporter 2

  17. Here’s this crazy piece on excessive bicarbonate - Gas production after reaction of sodium bicarbonate and hydrochloric acid

  18. Josh points out that the pH can be important for inotropy:  An effect of pH upon epinephrine inotropic receptors in the turtle heart

  19. Mel’s favorite from Halperin because of the pun: Renal tubular acidosis (RTA): recognize the ammonium defect and pHorget the urine pH

  20. Amy’s VOG on RTA and Osteoporosis

    1. KI Review on acidosis and bone health: Effects of acid on bone

    2. Guideline on congenital RTA: Distal renal tubular acidosis: ERKNet/ESPN clinical practice points

    3. AJKD article on acidosis and bone health: Serum Bicarbonate and Bone Mineral Density in US Adults

    4. Citrate reversing CsA induced acidosis effects: Citrate reverses cyclosporin A-induced metabolic acidosis and bone resorption in rats

Outline: Chapter 19 Metabolic Acidosis part 3

  • Renal Tubular Acidosis

    • Acidosis from diminished net tubular acid secretion

    • Three types

      • Type 1 (Distal)

      • Type 2 (Proximal)

      • Type 4 (…)

    • The acidosis of renal failure could be added to this group

      • But NH4+ per nephron is normal

        • This is a problem of too few nephrons, not tubular acidosis

          • Nephrons able to maximally acidify the urine

    • Type 1 Distal RTA

      • Decrease in net H secretion in the collecting duct

      • Minimal urine pH rises from 4.5 to 5.3

      • HCO3 can fall below 10

      • Three mechanisms

        • Defect in H-ATPase found in cortex and medulla

          • Sjögren syndrome

          • Can be genetic chloride bicarbonate exchanger

            • This pumps bicarbonate out basolateral membrane after it is generated in the splitting of water to form H

        • Defect in cortical Na reabsorption

          • Voltage-dependent defect

          • Concurrent K secretion defect

          • Found in urinary obstruction and sickle cell

          • Volume deficiency can decrease Na delivery to distal nephron

            • Decreased amount of Na reabsorption can cause a reversible type 1 RTA of this type

        • Increased membrane permeability

          • Amphotericin

          • pH of 5.0 is 250× plasma

          • Table 19-7

      • Fractional excretion of bicarbonate in distal RTA

        • Normally negligible since bicarbonate can’t exist with pH down around 5

        • In distal RTA it may be as high as 6.5; FEHCO3 is 3%

        • If pH goes up over 7 this can rise to 5–10%

          • Usually in infants

          • As they age their urine pH falls a bit

          • This is called type 3

      • Plasma K

        • H-ATPase defects have low K

          • Patients also have downregulation of H-K-ATPase

          • Downregulation of NaCl reabsorption in proximal tubule

            • Decreased filtered bicarbonate means less bicarbonate to absorb with Na, hence more Na excretion from proximal tubule

            • This increases distal sodium delivery and increases aldosterone

        • Voltage defect also has decreased renal K clearance → hyperkalemia

          • Differentiate from type 4 RTA by looking at urine pH

            • Lower in type 4

            • Higher in voltage-dependent distal RTA

      • Nephrocalcinosis

        • Hypercalciuria, hyperphosphatemia, nephrolithiasis, and nephrocalcinosis are frequent

        • Comes from bones buffering the acidosis

        • Kidney decreases reabsorption of these so they are lost in urine

        • Two other factors

          • Low urinary citrate

            • Hypokalemia drives this

            • Acidosis drives this

          • High urine pH (CaPhos stones)

        • All corrected by correcting the metabolic acidosis

      • Incomplete Type 1

        • Defective urinary acidification but not acidemic

        • Increased proximal NH3 production lowers urinary H

        • Low urinary citrate

        • Can progress to complete type 1

      • Etiology of Type 1

        • Sjögren syndrome, rheumatoid arthritis

        • 19-8

      • Clinical manifestations

        • Stones

        • Hypokalemia

        • Growth defects

      • Diagnosis

        • NAGMA and elevated urine pH

          • 5.3 in adults

          • 5.6 in children

        • Differentiate Type 1 vs Type 2

          • Give bicarbonate drip

            • 1 mEq/kg/hr

            • Urine pH remains high with Type 1

            • Does not go up as it does with proximal Type 2

        • Incomplete distal RTA

          • Give acid load

          • 0.1 mmol/kg

          • Urine pH remains >5.3 in classic

          • Falls in normal patients (usually below 5)

      • Treatment

        • Treat metabolic acidosis

          • Minimize potassium loss

          • Reduce bone catabolism

          • Prevent stones

        • Alkali requirement

          • Adults: 1–2 mEq/kg/day

          • Children: 4–14 mEq/kg/day

        • Alkali

          • Sodium bicarbonate

          • Sodium citrate

          • Potassium citrate if hypokalemia persists despite correcting acidosis

            • Or for calcium stone disease

        • Treat hypokalemia

    • Type 2 Proximal RTA

      • Decreased HCO3 reabsorption

      • 90% of bicarbonate reabsorption happens in proximal tubule

      • Bicarbonate wasting starts normally at 26–28 mmol/L (Tm for bicarbonate)

      • In RTA 2 the Tm falls to a lower level (maybe 17)

        • Serum bicarbonate falls to 17 and stabilizes

        • Type 2 RTA is self-limiting

        • Typically HCO3 around 14–20

      • Distal acidification intact

        • Carbonic anhydrase inhibitor can block 80% of proximal HCO3 reabsorption

        • Only 30% of filtered bicarbonate excreted due to distal H secretion

        • Total absence of proximal reabsorption results in HCO3 11–12

      • Clinical difference in treatment

        • In Type 2, giving bicarbonate and raising serum HCO3 above Tm → more wasted in urine

          • FEHCO3 can reach 15% with normal serum HCO3

          • Urine pH >7.5

        • Below Tm, urine pH <5.3

        • In Type 1, curve relating HCO3 excretion to plasma HCO3 similar to normal (with increased obligatory urine HCO3 due to higher urine pH)

      • Defect in HCO3 reabsorption

        • Can be isolated

        • Or part of Fanconi syndrome

      • Pathogenesis (three steps)

        • Na-H exchange (apical membrane)

        • Na-K-ATPase (basolateral membrane)

        • Carbonic anhydrase

          • Intracellular

          • Luminal

        • Multiple myeloma most common adult cause

        • Ifosfamide

          • Can also cause phosphate wasting, NDI, and Type 1 RTA

      • K balance

        • Common but variable

        • Mild hypokalemia at baseline due to increased Na wasting → hyperaldosteronism

        • Worse with bicarbonate therapy

          • Distal delivery of nonreabsorbable anion increases obligate cation loss

        • Figure 19-7

      • Bone disease

        • Rickets (children), osteomalacia/osteopenia (adults)

          • Up to 20%

          • Phosphate wasting and vitamin D deficiency may contribute

        • Impaired growth

        • No nephrocalcinosis or nephrolithiasis

          • Lower urine pH

          • Nonreabsorbable amino acids and organic anions bind calcium

      • Etiology

        • 19-9

        • Idiopathic and cystinosis (children)

        • Carbonic anhydrase inhibitors

        • Multiple myeloma

      • Diagnosis

        • NAGMA and pH <5.3

        • Look for Fanconi syndrome

        • Raise serum HCO3 and watch urine pH rise

          • FEHCO3 15–20%

      • Treatment

        • Correct acidosis to allow normal growth

          • Difficult due to rapid urinary loss

          • May need 10–15 mEq/kg/day

          • HCO3 or citrate

            • More than 20 mEq HCO3 can cause stomach rupture from CO2 generation

          • Small dose thiazide to increase proximal Na reabsorption and HCO3 reabsorption

        • Idiopathic Type 2 may improve after years

    • Type 4 RTA

      • Aldosterone deficient or resistant

      • Normally stimulates H secretion and K secretion

      • Loss causes hyperkalemia and metabolic acidosis

      • Hyperkalemia antagonizes NH4 generation

        • High K may outcompete NH4 on Na-K-2Cl in TALH

          • Less ammonium recycling

          • Less NH3 available in collecting duct

        • Correcting hyperkalemia can correct acidosis

      • Metabolic acidosis generally mild

        • HCO3 >15

        • Urine pH <5.3 (generally, not always)

        • Mineralocorticoid can treat but causes hypertension and sodium retention

        • Often responds to loop diuretic

  • Rhabdomyolysis can cause high anion gap metabolic acidosis

  • Symptoms

    • Respiratory compensation increases 4–8 fold → dyspnea

    • pH <7.0–7.1

      • Fatal ventricular arrhythmias

      • Reduced cardiac contractility

        • Decreased response to inotropes

    • Neurological

      • Lethargy to coma

      • More related to CSF pH than plasma

      • Less neurologic symptoms than respiratory acidosis

        • BBB more permeable to CO2 than HCO3

    • Skeletal problems

    • Decreased growth

    • Kids/infants: anorexia, nausea, listlessness

  • Treatment

    • General principles

      • Correct with HCO3

        • No alkali required for lactic or ketoacidosis

      • Goal: pH >7.2

        • Equations on page 629 need “log”

        • Example: pH 7.1, pCO2 20, HCO3 6

          • Raise HCO3 to 8 if pCO2 stays 20

          • Raise to 10 if pCO2 rises

          • Paragraph “regardless…” highlights risks of bicarbonate

    • Bicarbonate deficit

      • Deficit = HCO3 space × HCO3 deficit per liter

      • HCO3 space

        • 50% body weight (normal)

        • 60% (mild–moderate acidosis)

        • 70% (severe, HCO3 <8–10)

      • Example: 70 kg, raise HCO3 6→10 using 0.7 space = 196 mEq

      • Rough guideline; does not account for ongoing acid production

        • Early large bump in bicarbonate

        • Drifts down as bicarbonate moves intracellularly

    • Plasma potassium

      • K depletion can cause metabolic acidosis

      • Metabolic acidosis increases K

      • “Normal” K may mask depletion (see DKA)

      • Beware correcting acidosis in hypokalemia

    • Heart failure

      • Bicarbonate comes with sodium load

      • Comment that bicarbonate moves into cell

        • But Na remains extracellular

      • Dialysis can be used